Heparin strongly induces soluble fms-Like tyrosine kinase 1 release in vivo and in vitro: brief report

Searle, Julia, Mockel, Martin, Gwosc, Stefanie, Datwyler, Saul A., Fatimunnisa, Qadri, Albert, Gesa I., Holert, Fabian, Isbruch, Annette, Klug, Lars, Muller, Dominik N., Dechend, Ralf, Muller, Reinhold, Vollert, Joern O., Slagman, Anna, Mueller, Christian, and Herse, Florian (2011) Heparin strongly induces soluble fms-Like tyrosine kinase 1 release in vivo and in vitro: brief report. Arteriosclerosis, Thrombosis and Vascular Biology, 31 (12). pp. 2972-2974.

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Abstract

Objective: Soluble fms-like tyrosine kinase 1 (sFlt1) is involved in the pathophysiology of preeclampsia and coronary artery disease. Because sFlt1 has a heparin-binding site, we investigated whether or not heparin releases sFlt1 from the extracellular matrix.

Methods and Results: We measured sFlt1 before and after heparin administration in 135 patients undergoing coronary angiography, percutanous coronary intervention, or both. sFlt1 was increased directly after heparin administration (from 254 to 13 440 pg/mL) and returned to baseline within 10 hours. Umbilical veins and endothelial cells treated with heparin released sFlt1. Heparinase I and III also increased sFlt1. Mice treated with heparin had elevated sFlt1 serum levels. Their serum inhibited endothelial tube formation.

Conclusion: Heparin releases sFlt1 by displacing the sFlt1 heparin-binding site from heparan sulfate proteoglycans. Heparin could induce an antiangiogenic state.

Item ID: 21428
Item Type: Article (Refereed Research - C1)
Keywords: angiogenesis, angiography, coronary artery disease, heparin, sFlt1
ISSN: 1524-4636
Date Deposited: 05 Apr 2012 04:11
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1117 Public Health and Health Services > 111799 Public Health and Health Services not elsewhere classified @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920199 Clinical Health (Organs, Diseases and Abnormal Conditions) not elsewhere classified @ 100%
Citation Count from Web of Science Web of Science 2
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