Peptidyl-tRNA hydrolase screening combined with molecular docking reveals the antibiotic potential of Syzygium johnsonii bark extract
Harris, Sarah M., McFeeters, Hana, Ogungbe, Ifedayo, Cruz-Vera, Louis R, Setzer, William, Jackes, Betsy, and McFeeters, Robert L (2011) Peptidyl-tRNA hydrolase screening combined with molecular docking reveals the antibiotic potential of Syzygium johnsonii bark extract. Natural Product Communications, 6 (10). pp. 1421-1424.
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With the rapid rise of antibiotic resistance in pathogenic bacteria, the need for new antibacterial agents is overwhelming. Herein we report the limited screening of tropical plant extracts for inhibitory activity against the essential enzyme peptidyl-tRNA hydrolase (Pth). Initial screening was conducted through an electrophoretic mobility assay and Northern blot detection. The ability of Pth to cleave the peptide-tRNA ester boud was assessed. The ethanol bark extract of Syzygium johnsonii showed strong inhibitory potential. Molecular docking studies point to Syzygium polyphenolics as the potential source of inhibition. This work is the forerunner of activity-directed isolation, purification, and structure elucidation of the inhibitory components from Syzygium johnsonii extracts and studies of compound interaction with Pth.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||antibacterial; tropical plant extracts; Syzygium johnsonii; peptidyl-tRNA hydrolase; molecular docking; phytochemical screening|
|Date Deposited:||04 Apr 2012 05:02|
|FoR Codes:||05 ENVIRONMENTAL SCIENCES > 0502 Environmental Science and Management > 050202 Conservation and Biodiversity @ 100%|
|SEO Codes:||82 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 8299 Other Plant Production and Plant Primary Products > 829999 Plant Production and Plant Primary Products not elsewhere classified @ 100%|
|Citation Count from Web of Science||