2D and 3D mapping of microindentations in hydrated and dehydrated cortical bones using confocal laser scanning microscopy

Yin, Ling, Venkatesan, Sudharshan, Webb, Daryl, Kalyanasundaram, Shankar, and Qin, Qing-Hua (2012) 2D and 3D mapping of microindentations in hydrated and dehydrated cortical bones using confocal laser scanning microscopy. Journal of Materials Science, 47 (10). pp. 4432-4438.

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Abstract

We report on responses of hydrated and dehydrated cortical bone tissues to mechanical loading applied by a Vickers indenter. The Vickers indentations were imaged in two- and three-dimensions (2D and 3D) using confocal laser scanning microscopy (CLSM) to understand mechanical behavior of bone tissues. Serial optical sections of indentation patterns of dry and wet bones were collected using CLSM. The indention surface structures were mapped using topographical CLSM imaging. The observation of CLSM shows the fundamental indentation responses for both the hydrated and dehydrated bone tissues were plastic deformation. No visible fracture was observed in the Vickers indentation patterns in the wet bone tissue, while non-propagating lamellar microcracks occurred in the dry bone tissue. This indicates that drying resulted in increased brittleness of the bone tissue. The Vickers hardness values of dry bone tissue were significantly higher than those of wet bone tissue at any applied loads (analysis of variation, ANOVA, p < 0.05). The resolution limits of confocal microscopy were also discussed for bone tissue scanning.

Item ID: 20968
Item Type: Article (Refereed Research - C1)
Additional Information:

This study was presented in part at the 6th Australasian Congress on Applied Mechanics, ACAM 6, 12–15 Dec 2010, Perth, Australia.

ISSN: 1573-4803
Date Deposited: 05 Apr 2012 04:20
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1105 Dentistry > 110501 Dental Materials and Equipment @ 50%
09 ENGINEERING > 0903 Biomedical Engineering > 090302 Biomechanical Engineering @ 50%
SEO Codes: 92 HEALTH > 9204 Public Health (excl. Specific Population Health) > 920402 Dental Health @ 100%
Citation Count from Web of Science Web of Science 2
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