Identification of seventeen microsatellite markers for conservation genetic studies of the endemic anemonefish, Amphiprion mccullochi

van der Meer, Martin H., Gardiner, Michael G., Hobbs, Jean-Paul A., Jones, G.P., and van Herwerden, Lynne (2012) Identification of seventeen microsatellite markers for conservation genetic studies of the endemic anemonefish, Amphiprion mccullochi. Conservation Genetics.

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Abstract

Endemic species at remote islands have a high risk of extinction because they often exhibit ecological, biological and genetic traits that make them particularly vulnerable to disturbances. McCulloch's anemonefish (Amphiprion mccullochi) is endemic to a few oceanic reefs off Australia's east coast and is an habitat specialist. Using 454 shotgun sequencing, we developed and evaluated primers for seventeen independent microsatellite loci to reveal gene flow, population genetic structure and genetic diversity across three isolated reefs. Observed and expected heterozygosities ranged from 0.556 to 1 and 0.543 to 0.907, respectively, in 30 Lord Howe Island individuals. When cross tested with the close relative, Amphiprion akindynos, amplification was successful with high levels of polymorphism. These loci will therefore be useful in studies of A. mccullochi, A. akindynos and possibly other closely related anemonefish.

Item ID: 19587
Item Type: Article (Refereed Research - C1)
Keywords: coral reef fish, island endemics, genetic diversity, cross-species amplification
ISSN: 1572-9737
Date Deposited: 16 Jan 2012 04:18
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
Citation Count from Web of Science Web of Science 2
Downloads: Total: 1
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