Biochemical characterization of plasma membrane vesicles of Cyanophora paradoxa

Heimann, Kirsten, Becker, B., Harnisch, Helga, Mukherjee, K.D., and Melkonian, M. (1997) Biochemical characterization of plasma membrane vesicles of Cyanophora paradoxa. Botanica Acta, 110. pp. 401-410.

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Abstract

Plasma membrane vesicles (PMVs) were isolated PA: from Cyanophora paradoxa Korshikov (Glaucocystophyta) and purified by differential and sucrose density gradient centrifugation PMVs were identified ultrastructurally and biochemically using marker enzymes. Analysis of monosaccharides revealed a sugar content of 37.3% of dry weight of delipidated PMVs with high amounts of fucose (28 mol% of total carbohydrate), galactose (23%), mannose (21%) and the minor constituents xylose, rhamnose, glucose and N-acetylglucosamine Proteins were analyzed by SDS-PAGE. More than 20 polypeptides were resolved and several were identified as glycopeptides, as shown by PAS staining and lectin blots. Fucose linked alpha-1-->6 to N-acetylglucosamine (AAA-binding), mannose linked alpha-1-->2,3,6 to mannose (GNA-binding) and terminally bound galactose (RCA-binding) were identified on several polypeptides. Sterols (p-sitosterol and an additional unknown sterol, 40% of total lipids) and diacylglycerol (43%) are the major lipid components in the PMVs of C. paradoxa. Minor components are PE, PC, PG and an unknown glycolipid. Fatty acid analysis revealed 12 different fatty acids, predominantly 16:0, 18:0 and the polyunsaturated acids 20:4 and 20:5 (together 69% of total fatty acids). alpha-Linolenic acid, common in higher plant PMs, could only be detected as a minor constituent (< 1 %) of PMVs of C. paradoxa.

Item ID: 1935
Item Type: Article (Refereed Research - C1)
Keywords: algae, glycoproteins, lipids, fatty acids, plasma membrane, Cyanophora paradoxa
ISSN: 1438-8677
Date Deposited: 30 Jul 2008
FoR Codes: 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060110 Receptors and Membrane Biology @ 0%
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