Estimating sample sizes for DNA barcoding

Zhang, A.B., He, L.J., Crozier, R.H., Muster, C., and Zhu, C-D. (2010) Estimating sample sizes for DNA barcoding. Molecular Phylogenetics and Evolution, 54 (3). pp. 1035-1039.

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Abstract

Sample size has long been one of the basic issues since the start of the DNA barcoding initiative and the global biodiversity investigation. As a contribution to resolving this problem, we propose a simple resampling approach to estimate several key sampling sizes for a DNA barcoding project. We illustrate our approach using both structured populations simulated under coalescent and real species of skipper butterflies. We found that sample sizes widely used in DNA barcoding are insufficient to assess the genetic diversity of a species, population structure impacts the estimation of the sample sizes, and hence will bias the species identification potentially.

Item ID: 16988
Item Type: Article (Refereed Research - C1)
Keywords: DNA barcoding, sampling sizes, non-parametric resampling, statistical approach
ISSN: 1095-9513
Date Deposited: 15 May 2011 10:46
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060409 Molecular Evolution @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
Citation Count from Web of Science Web of Science 2
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