Circulating concentrations of stem-cell-mobilizing cytokines are associated with levels of osteoprogenitor cells and aortic calcification severity

Pal, Shripad N., Clancy, Paula, and Golledge, Jonathan (2011) Circulating concentrations of stem-cell-mobilizing cytokines are associated with levels of osteoprogenitor cells and aortic calcification severity. Circulation Journal, 75 (5). pp. 1227-1234.

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Abstract

Background: To investigate the association between aortic calcification, concentrations of stem-cell-mobilizing cytokines and osteocalcin-positive mononuclear cells in a mouse model and patients with peripheral artery disease.

Methods and Results: We estimated the concentration of the stem-cell-mobilizing cytokines stromal cell-derived factor α (SDF-1α), granulocyte colony stimulating factor and stem cell factor in a mouse model of aortic calcification developed in osteoprotegerin-deficient (OPG-/-) mice, as well as in patient plasma samples. Calcification was estimated by a colorimetric assay of extracts of harvested mice aortas and by computed tomographic angiogram images in patients. The cytokine concentrations were assessed for association with the severity of calcification and the percentage of osteocalcin-positive mononuclear cells (OCN+ MNC) using non-parametric analysis. The serum concentration of stromal SDF-1α and granulocyte-colony stimulating factor (G-CSF) were significantly greater in OPG-/- compared to control mice. The percentage of circulating OCN+ MNC was correlated to the concentration of SDF-1α in OPG-/- mice. These cytokines also correlated with the severity of calcification in OPG-/- mice. Patients with more severe calcification had a higher plasma concentration of the cytokines than those with less marked aortic calcification. The concentrations also correlated with circulating OCN+ MNC and aortic calcification volumes.

Conclusions: The association between stem cell cytokines, OCN+ MNC and calcification suggests a possible role of bone-derived osteoprogenitor cells in the pathogenesis of vascular calcification.

Item ID: 16881
Item Type: Article (Refereed Research - C1)
Keywords: cytokines; peripheral artery disease; stem cells; vascular calcification
ISSN: 1347-4820
Date Deposited: 27 Jun 2011 06:42
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1102 Cardiovascular Medicine and Haematology > 110201 Cardiology (incl Cardiovascular Diseases) @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920103 Cardiovascular System and Diseases @ 100%
Citation Count from Web of Science Web of Science 2
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