The relationship between collagen scaffold cross-linking agents and neutrophils in the foreign body reaction
Ye, Qingsong, Harmsen, Martin C., van Luyn, Marja J.A., and Bank, Ruud A. (2010) The relationship between collagen scaffold cross-linking agents and neutrophils in the foreign body reaction. Biomaterials, 31 (35). pp. 9192-9201.
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In order to get more insight into the role of neutrophils on the micro-environment and consequently on macrophages in the foreign body reaction in mice, we investigated the fate of the two differently cross-linked dermal sheep collagen disks (glutaraldehyde = GDSC, hexamethylenediisocyanate = HDSC) in mice implanted in one anatomical location, namely subcutaneously. In GDSC massive infiltration of neutrophils is seen at day 2 and day 21, whereas in HDSC only minor infiltration is seen at day 2. The presence of neutrophils coincided with high levels of IFN-γ, a cytokine that activates macrophages. Major differences were seen in degradation rate of the two disks: GDSC was almost completely degraded after 28 days, whereas HDSC remained intact. Degradation of GDSC occurred through collagenolytic activity and phagocytosis by macrophages. Phagocytosis was observed at day 2 and day 21. IL-13 was only observed in HDSC, and this resulted in the presence of giant cells in HDSC. These giant cells produced IL-10, that promoted TIMP-1 expression and that inhibits collagenolytic and phagocytic activity. We conclude that the function of macrophages in mice is largely influenced by differences in micro-environment induced by GDSC and HDSC and that the presence/absence of neutrophils play a major role in the shaping of this micro-environment.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||marix metalloproteinase, foreign body reaction, giant cell formation, phagocytosis, interferon-gamma|
|Date Deposited:||29 Apr 2011 06:46|
|FoR Codes:||09 ENGINEERING > 0903 Biomedical Engineering > 090301 Biomaterials @ 100%|
|SEO Codes:||97 EXPANDING KNOWLEDGE > 970111 Expanding Knowledge in the Medical and Health Sciences @ 100%|
|Citation Count from Web of Science||