Differential effects of temperature and maturity stage on hepatic estrogen receptor characteristics of Atlantic salmon

Watts, M., Pankhurst, N.W., King, H.R., and Geraghty, D.P. (2005) Differential effects of temperature and maturity stage on hepatic estrogen receptor characteristics of Atlantic salmon. Comparative Biochemistry and Physiology-Part A: molecular & integrative physiology, 140 (3). pp. 377-383.

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Abstract

In order to determine if elevated temperature during vitellogenesis had a detrimental effect on hepatic estrogen receptors of Atlantic salmon (Salmo salar), 3H-estradiol saturation binding analysis, using one- and two-site binding models, was carried out on extracts of hepatic cytosols from fish held at 14, 18 or 22 °C over the austral period of peak vitellogenesis (February to April). With one-site binding analysis, no temperature related difference in either receptor affinity (Kd) or number (Bmax) was found at each sampling point, but there was an apparent decrease in both affinity and number at each temperature over the period of the study. However, some analyses, notably at 22 °C during February, were best described using a two-site binding model. At this temperature and time, there was a clear separation of binding affinity into high and low components (Kd=0.67±S.E. 0.05 and 20±S.E. 5.6 nM, respectively) (n=4), which suggests that February was a critical time of temperature related hepatic sensitivity to estrogen. These results support those of other studies where we found that February was also a sensitive time with respect to temperature impairment of in vitro follicular estrogen synthesis, and the greatest period of in vivo temperature sensitivity.

Item ID: 13998
Item Type: Article (Refereed Research - C1)
Keywords: Atlantic salmon; saturation binding assays; estrogen receptors; temperature
ISSN: 1531-4332
Date Deposited: 23 Nov 2010 23:52
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 100%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830199 Fisheries - Aquaculture not elsewhere classified @ 100%
Citation Count from Web of Science Web of Science 8
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