Stimulation of renal sulfate secretion by metabolic acidosis requires Na+/H+ exchange induction and carbonic anhydrase
Pelis, Ryan M., Edwards, Susan L., Kunigelis, Stan C., Claiborne, James B., and Renfro, J. Larry (2005) Stimulation of renal sulfate secretion by metabolic acidosis requires Na+/H+ exchange induction and carbonic anhydrase. American Journal of Physiology: renal physiology, 289 (3). pp. 208-216.
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The acute effect of metabolic acidosis on SO42– secretion by the marine teleost renal proximal tubule was examined. Metabolic acidosis was mimicked in primary cultures of winter flounder renal proximal tubule epithelium (fPTCs) mounted in Ussing chambers by reducing interstitial pH to 7.1 (normally 7.7). fPTCs with metabolic acidosis secreted SO42– at a net rate that was 40% higher than in paired isohydric controls (pH 7.7 on interstitium). The stimulation was completely blocked by the carbonic anhydrase inhibitor methazolamide (100 µM). Although Na+/H+ exchange (NHE) isoforms 1, 2, and 3 were identified in fPTCs by immunoblotting, administering EIPA (20 µM) to the interstitial and luminal bath solutions had no effect on net SO42– secretion by fPTCs with a normal interstitial pH of 7.7. However, EIPA (20 µM) blocked most of the stimulation caused by acidosis when applied to the lumen but not interstitium, demonstrating that induction of brush-border NHE activity is important. In the intact flounder, serum pH dropped 0.4 pH units (pH 7.7 to 7.3, at 2–3 h) when environmental pH was lowered from 7.8 to ~4.3. Whereas serum [SO42–] was not altered by acidosis, renal tubular SO42– secretion rate was elevated 200%. Thus metabolic acidosis strongly stimulates renal sulfate excretion most likely by a direct effect on active renal proximal tubule SO42– secretion. This stimulation appears to be dependent on inducible brush-border NHE activity.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||epithelial transport; marine teleost; metalbolic alkalosis; proximal tubule; transport metabolon|
|Date Deposited:||25 Nov 2010 04:50|
|FoR Codes:||07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070702 Veterinary Anatomy and Physiology @ 34%
06 BIOLOGICAL SCIENCES > 0606 Physiology > 060603 Animal Physiology Systems @ 33%
06 BIOLOGICAL SCIENCES > 0606 Physiology > 060604 Comparative Physiology @ 33%
|SEO Codes:||96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960802 Coastal and Estuarine Flora, Fauna and Biodiversity @ 34%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 33%
93 EDUCATION AND TRAINING > 9399 Other Education and Training > 939999 Education and Training not elsewhere classified @ 33%
|Citation Count from Scopus||