Purification of fully activated Clostridium botulinum serotype B toxin for treatment of patients with dystonia

Arimitsu, Hideyuki, Inoue, Kaoru, Sakaguchi, Yoshihiko, Lee, Jaechul, Fujinaga, Yukako, Watanabe, Toshihiro, Ohyama, Tohru, Hirst, Robert, and Oguma, Keiji (2003) Purification of fully activated Clostridium botulinum serotype B toxin for treatment of patients with dystonia. Infection and Immunity, 71 (3). pp. 1599-1603.

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Abstract

Clostridium botulinum serotype B toxins 12S and 16S were separated by using a beta-lactose gel column at pH 6.0; toxin 12S passed through the column, whereas toxin 16S bound to the column and eluted with lactose. The fully activated neurotoxin was obtained by applying the trypsin-treated 16S toxin on the same column at pH 8.0; the neurotoxin passed through the column, whereas remaining nontoxic components bound to the column. The toxicity of this purified fully activated neurotoxin was retained for a long period by addition of albumin in the preparation.

Item ID: 13759
Item Type: Article (Refereed Research - C1)
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ISSN: 1070-6313
Date Deposited: 29 Nov 2010 03:11
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1199 Other Medical and Health Sciences > 119999 Medical and Health Sciences not elsewhere classified @ 100%
SEO Codes: 92 HEALTH > 9299 Other Health > 929999 Health not elsewhere classified @ 100%
Citation Count from Web of Science Web of Science 2
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Last 12 Months: 1
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