Multiplication of the V4 strain of Newcastle disease virus in Madin Derby bovine kidney cells
Sahle, M., Burgess, W.G., and Kideanemariam, A. (2002) Multiplication of the V4 strain of Newcastle disease virus in Madin Derby bovine kidney cells. Onderstepoort Journal of Veterinary Research, 69 (3). pp. 201-206.
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This study describes a reproducible cell culture system that permits the growth and secondary multiplication of the V4 strain of Newcastle disease virus. Allantoic fluid, magnesium chloride and diethylaminoethyl dextran were incorporated in Dulbecco's modified Eagle's medium to encourage secondary viral multiplication without adversely affecting healthy Madin Derby bovine kidney cell growth.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||cell culture; MDBK cell; Newcastle disease virus; V4 strain replication|
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|Date Deposited:||06 Dec 2010 07:04|
|FoR Codes:||07 AGRICULTURAL AND VETERINARY SCIENCES > 0702 Animal Production > 070205 Animal Protection (Pests and Pathogens) @ 100%|
|SEO Codes:||83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8303 Livestock Raising > 830309 Poultry @ 100%|