Mucosal immunisation with Clostridium botulinum type C 16 S toxoid and its non-toxic component

Mahmut, Nazira, Inoue, Kaoru, Fujinaga, Yukako, Arimitsu, Hideyuki, Sakaguchi, Yoshihiko, Hughes, Lynn, Hirst, Robert, Murphy, Tom, Tsuji, Takao, Watanabe, Toshihiro, Ohyama, Tohru, Karasawa, Tadahiro, Nakamura, Shinichiro, Yokota, Kenji, and Oguma, Kenji (2002) Mucosal immunisation with Clostridium botulinum type C 16 S toxoid and its non-toxic component. Journal of Medical Microbiology, 51. pp. 813-820.

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Abstract

Clostridium botulinum types C and D produce a 16 S (500 kDa) toxin that is formed by conjugation of neurotoxin with a non-toxic component (nonTox). The amino acid sequences of type C and D nonTox components are almost identical. In a previous report it was proposed that nonTox is necessary for the effective absorption of the toxin from the small intestine. This suggested the hypothesis that mucosal immunity against nonTox in the small intestine might prevent the absorption of both C- and D-16 S toxins. The nonTox was purified from a mutant strain, (C)-N71, that does not produce neurotoxin. This nonTox or detoxified C-16 S toxin were mixed with adjuvant (a mutant form of heat-labile toxin of Escherichia coli), and inoculated into mice via the nasal or oral route, or both. The mice inoculated nasally four times with nonTox or toxoid produced high levels of antibodies (including IgA) against the immunogens, both in intestinal fluids and sera. When these nonTox-immunised mice were challenged orally with 2 and 20 oral minimum lethal doses (MLD) of C- or D-16 S toxins, the same results were obtained with both C and D; the mice survived after challenge with 2 MLD of either C or D but were killed by 20 MLD of either toxin although the time to death was significantly longer than in the control non-immunised mice. These results indicate that the local anti-nonTox antibodies reduce absorption of both C- and D-16 S toxins from the small intestine. The C-16 S toxoid-immunised mice showed similar behaviour with type D toxin challenge, probably due to the same mechanism, but were protected against 20 MLD of C-16 S toxin.

Item ID: 13585
Item Type: Article (Refereed Research - C1)
Keywords: Clostridium botulinum; ELISA
ISSN: 0022-2615
Date Deposited: 17 Nov 2010 00:09
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060501 Bacteriology @ 51%
11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110801 Medical Bacteriology @ 49%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy @ 51%
92 HEALTH > 9202 Health and Support Services > 920205 Health Education and Promotion @ 49%
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