A cost-effective method of preparing larval fish otoliths for reading using enzyme digestion and staining

Green, B.S., Reilly, S.M., and McCormick, M.I. (2002) A cost-effective method of preparing larval fish otoliths for reading using enzyme digestion and staining. Journal of Fish Biology, 61 (6). pp. 1600-1605.

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Abstract

A fast and cost-effective method for examining otoliths in fish larvae was developed whereby the otolith remains in situ. Whole fish of the clownfish Amphiprion melanopus were enzymecleared using a laundry pre-soak and then stained using the Von Kossa silver staining method for calcium. The otolith nucleus, daily rings and the otolith edge were all clearly visible and were suitable for a variety of age and growth analyses. The total ‘hands on’ time required to process these otoliths was c. 3 min, and multiple samples could be processed simultaneously. The reduction in labour of this method to produce clear daily rings in the otolith lends itself to broad use in fish biology where large quantities of otoliths need to be examined in a cost- and time-efficient manner.

Item ID: 13572
Item Type: Article (Refereed Research - C1)
Keywords: Amphiprion melanopus; daily increments; fish larvae; otoliths
ISSN: 1095-8649
Date Deposited: 08 Dec 2010 05:50
FoR Codes: 06 BIOLOGICAL SCIENCES > 0602 Ecology > 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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