The complete nucleotid sequence of the Vibrio harveyi bacteriophage VHML
Oakey, H.J., Cullen, B.R., and Owens, L. (2002) The complete nucleotid sequence of the Vibrio harveyi bacteriophage VHML. Journal of Applied Microbiology, 93 (6). pp. 1089-1098.
PDF (Published Version)
Restricted to Repository staff only
Aims: To determine the complete nucleotide sequence of the bacteriophage VHML and establish a hypothesis for the virulence conversion caused by VHML infection of Vibrio harveyi.
Methods and Results: The complete nucleotide sequence of VHML was determined (43 193 bp) and used to identify putative genes. The translated products of these genes were compared with reported sequences to assign hypothetical functions. All anticipated structural genes and putative genes for lysogeny were identified. In addition, we found a complete N6-adenine methyltransferase (Dam) gene that appeared to have an essential site for ADP-ribosylating toxins at the C-terminal of the translated product.
Conclusions: Virulence conversion of V. harveyi by VHML may be associated with Dam transcriptional regulation. The Dam gene may also encode for a toxin component similar to ADP-ribosylating toxins.
Significance and Impact of Study: This manuscript lays the foundation for understanding the virulence of toxin-producing V. harveyi. Further research into aspects discussed here will lead to a greater comprehension regarding the invertebrate disease vibriosis and its control in the farming of these animals.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||bacteriophage VHML; nucleotide sequence; Penaeus monodon; Vibrio harveyi; virulence|
|Date Deposited:||04 Dec 2010 13:02|
|FoR Codes:||07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 51%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070404 Fish Pests and Diseases @ 49%
|SEO Codes:||83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830199 Fisheries - Aquaculture not elsewhere classified @ 100%|