DNA sequence variation in the ITS-I rDNA subunit and host relationships in sorghum midge, Stenodiplosis sorghicola (Coquillett) (Diptera: Cediomyiidae), in Australia
Congdon, Bradley C., Kurniasih, , Franzmann, Bernard A., and Hardy, Adam T. (2002) DNA sequence variation in the ITS-I rDNA subunit and host relationships in sorghum midge, Stenodiplosis sorghicola (Coquillett) (Diptera: Cediomyiidae), in Australia. Australian Journal of Entomology, 41 (2). pp. 106-110.
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Sequence variation in the internal transcribed spacer (ITS-1) ribosomal DNA subunit was examined for sorghum midge obtained from introduced and native hosts in south-eastern and central Queensland. No variation was observed relative to host plant or geographical distance for midges collected from two introduced hosts, grain sorghum (Sorghum bicolor) and Johnson grass (S. halepense); however, sequence differences were observed between midges from introduced and native hosts and among midges from a single native host, slender bluegrass (Dichanthium affine). No evidence was observed of introduced midges on native hosts, or vice versa. These results agree with previously hypothesised host distributions for native and introduced midges in Australia, and expand the sample of introduced hosts to include Johnson grass. They suggest that Stenodiplosis sorghicola, the principal midge infesting grain sorghum, is also the most common species on Johnson grass. This confirms that Johnson grass plays a role in the population dynamics of S. sorghicola and suggests that midges originating from Johnson grass may influence levels of infestation in grain sorghum.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||exotic species management; host-plant range; phylogenetic analysis|
|Date Deposited:||15 Dec 2010 03:08|
|FoR Codes:||06 BIOLOGICAL SCIENCES > 0699 Other Biological Sciences > 069999 Biological Sciences not elsewhere classified @ 100%|
|SEO Codes:||96 ENVIRONMENT > 9604 Control of Pests, Diseases and Exotic Species > 960499 Control of Pests, Diseases and Exotic Species not elsewhere classified @ 100%|