Microsatellite markers in the primitive termite Mastotermes darwiniensis

Goodisman, Michael A.D., Evans, Theodore A., Ewen, John G., and Crozier, Ross H. (2001) Microsatellite markers in the primitive termite Mastotermes darwiniensis. Molecular Ecology Resources, 1 (4). pp. 250-251.

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Abstract

We developed 10 microsatellite loci in the primitive termite Mastotermes darwiniensis. The number of alleles per locus ranged from four to 15, and the expected heterozygosites spanned from 0.21 to 0.90, in a sample of 40 workers collected from the Northern Territory, Australia. We also determined that only two loci amplified in five other termite species. The low frequency of cross-amplification probably resulted from the high level of phylogenetic divergence between M. darwiniensis and the other taxa. Thus, although the loci are not widely applicable, they should prove effective in elucidating the genetic structure of M. darwiniensis populations.

Item ID: 13348
Item Type: Article (Refereed Research - C1)
Keywords: gene flow; Isoptera; population genetic structure; relatedness; single locus DNA markers; social insect
ISSN: 1755-0998
Date Deposited: 30 Aug 2012 22:42
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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