Inhibition of growth of cultured Babesia microti by serum and macrophages in the presence or absence of T cells

Chen, Dehua, Copeman, D. Bruce, Hutchinson, Gareth W., and Burnell, Jim (2000) Inhibition of growth of cultured Babesia microti by serum and macrophages in the presence or absence of T cells. Parasitology International, 48 (3). pp. 223-231.

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Abstract

Serum and macrophages from the acute-phase (days 12–14 p.i.) and recovery-phase (days 23–25 p.i.) of infection of mice with Babesia microti were analyzed for their ability to inhibit the in vitro growth of B. microti in the presence or absence of T cells. Recovery-phase serum was inhibitory to the growth of B. microti, whereas, acute-phase serum had no inhibitory effects. Both acute- and recovery-phase macrophages inhibited B. microti growth. The co-culture of acute- but not recovery-phase T cells with macrophages from uninfected control mice was inhibitory to the growth of B. microti. Growth of B. microti was also inhibited in cultures containing macrophages from uninfected control mice plus culture supernatant fluid from acute-phase but not recovery-phase T cells. The supernatant fluid from B. microti cultures with acute-phase T cells contained IFN-γ detected by a sandwich ELISA, whereas cultures with control T cells or recovery phase T cells did not. Results of the present study suggest the likelihood of a protective role against B. microti in mice for antibody which appeared in recovery-phase serum and for macrophages activated by IFN-γ from acute-phase T cells.

Item ID: 12986
Item Type: Article (Refereed Research - C1)
Keywords: Babesia microti; mice; interferon-gamma; antibody; macrophages; T cells
ISSN: 1873-0329
Date Deposited: 22 Jul 2012 12:16
FoR Codes: 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060199 Biochemistry and Cell Biology not elsewhere classified @ 100%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8303 Livestock Raising > 830301 Beef Cattle @ 100%
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