CC chemokine ligand 20 partially controls adhesion of naive B cells to activated endothelial cells under shear stress
Meissner, Anja, Zilles, Olaf, Varona, Rosa, Jozefowski, Katrin, Ritter, Uwe, Marquez, Gabriel, Hallmann, Rupert, and Körner, Heinrich (2003) CC chemokine ligand 20 partially controls adhesion of naive B cells to activated endothelial cells under shear stress. Blood, 120 (8). pp. 2724-2727.
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Chemokines are thought to control lymphocyte recruitment to the inflamed endothelium. To dissect chemokine-mediated adhesion, binding of ex vivo isolated splenocytes to tumor necrosis factor (TNF)–activated endothelial cells was analyzed under shear stress. We observed specific adhesion of naive follicular B cells, which could be blocked by pertussis toxin. This indicated a G protein–mediated binding and pointed at a contribution of chemokine receptors to B-cell adhesion. Analysis of chemokines expressed by TNF-activated endothelial cells showed that CC chemokine ligand 2 (CCL2), CCL17, and CCL20 were up-regulated. Only on follicular B cells was the cognate receptor for CCL20, CC chemokine receptor 6 (CCR6), expressed strongly, and a functional transmigration assay with CCR6-negative B cells demonstrated conclusively the sole signaling of CCL20 through CCR6. Desensitization of CCR6 on naive B cells with CCL20 resulted in receptor down-regulation and reduced B-cell adhesion. We conclude that CCL20 plays a vital role in B-cell adhesion to the inflamed endothelium.
|Item Type:||Article (Refereed Research - C1)|
|Date Deposited:||26 Oct 2010 01:58|
|FoR Codes:||11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110707 Innate Immunity @ 100%|
|SEO Codes:||97 EXPANDING KNOWLEDGE > 970111 Expanding Knowledge in the Medical and Health Sciences @ 100%|