Rapid global expansion of the fungal disease chytridiomycosis into declining and healthy amphibian populations

James, Timothy Y., Litvintseva, Anastasia P., Vilgalys, Rytas, Morgan, Jess A.T., Taylor, John W., Fisher, Matthew C., Berger, Lee, Weldon, Ché, du Preez, Louis, and Longcore, Joyce E. (2009) Rapid global expansion of the fungal disease chytridiomycosis into declining and healthy amphibian populations. PLoS Pathogens, 5 (5). - .

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Abstract

The fungal disease chytridiomycosis, caused by Batrachochytrium dendrobatidis, is enigmatic because it occurs globally in both declining and apparently healthy (non-declining) amphibian populations. This distribution has fueled debate concerning whether, in sites where it has recently been found, the pathogen was introduced or is endemic. In this study, we addressed the molecular population genetics of a global collection of fungal strains from both declining and healthy amphibian populations using DNA sequence variation from 17 nuclear loci and a large fragment from the mitochondrial genome. We found a low rate of DNA polymorphism, with only two sequence alleles detected at each locus, but a high diversity of diploid genotypes. Half of the loci displayed an excess of heterozygous genotypes, consistent with a primarily clonal mode of reproduction. Despite the absence of obvious sex, genotypic diversity was high (44 unique genotypes out of 59 strains). We provide evidence that the observed genotypic variation can be generated by loss of heterozygosity through mitotic recombination. One strain isolated from a bullfrog possessed as much allelic diversity as the entire global sample, suggesting the current epidemic can be traced back to the outbreak of a single clonal lineage. These data are consistent with the current chytridiomycosis epidemic resulting from a novel pathogen undergoing a rapid and recent range expansion. The widespread occurrence of the same lineage in both healthy and declining populations suggests that the outcome of the disease is contingent on environmental factors and host resistance.

Item ID: 10963
Item Type: Article (Refereed Research - C1)
Keywords: chytridiomycosis, molecular epidemiology, phylogenetics
ISSN: 1553-7374
Date Deposited: 08 May 2010 02:16
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 40%
06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060504 Microbial Ecology @ 40%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070707 Veterinary Microbiology (excl Virology) @ 20%
SEO Codes: 96 ENVIRONMENT > 9605 Ecosystem Assessment and Management > 960505 Ecosystem Assessment and Management of Forest and Woodlands Environments @ 50%
96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960805 Flora, Fauna and Biodiversity at Regional or Larger Scales @ 50%
Citation Count from Web of Science Web of Science 76
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