Involvement of actin and microtubules in regulation of bioluminescence and translocation of chloroplasts in the dinoflagellate Pyrocystis lunula

Heimann, Kirsten, Klerks, Paul L., and Hasenstein, Karl H. (2009) Involvement of actin and microtubules in regulation of bioluminescence and translocation of chloroplasts in the dinoflagellate Pyrocystis lunula. Botanica Marina, 52 (2). pp. 170-177.

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Abstract

Bioluminescence in Pyrocystis lunula depends on circadian movement of scintillons and chloroplasts between active and inactive locations. The involvement of the cytoskeleton in these transport processes was investigated using cytoskeleton-specific drugs. Chloroplast movements were visualized by chlorophyll autofluorescence and confocal scanning laser microscopy. Latrunculin B and Oryzalin prevented translocation of chloroplasts. Four hours after transition to the night phase, mechanically inducible bioluminescence was inhibited by Latrunculin B, the myosin inhibitor 2,3 butanedione monoxime, and Oryzalin in dose-dependent fashions. The actin stabilizer Jasplakinolide slightly enhanced bioluminescence but eliminated Latrunculin B effects. The microtubule depolymerizer Colchicine had no effect on bioluminescence. These results suggest that F-actin and microtubules are involved in the movements of chloroplasts and scintillons. Although the drugs tested affected bioluminescence, further experiments are needed to determine whether effects on bioluminescence were mediated by drug-induced failed translocations of scintillons or chloroplasts.

Item ID: 10524
Item Type: Article (Refereed Research - C1)
Keywords: circadian rhythm; cytoskeleton; Latrunculin B; Oryzalin; scintillon
ISSN: 1437-4323
Date Deposited: 16 Apr 2010 04:38
FoR Codes: 06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060701 Phycology (incl Marine Grasses) @ 50%
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060199 Biochemistry and Cell Biology not elsewhere classified @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
Citation Count from Web of Science Web of Science 1
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