High resolution mapping of quantitative trait loci on ovine chromosome 3 and 20 affecting protein yield and lactation persistency
Singh, M., Lam, M., McGill, D., Thomson, P.C., Cavanagh, J.A., Zenger, K.R., and Raadsma, H.W. (2007) High resolution mapping of quantitative trait loci on ovine chromosome 3 and 20 affecting protein yield and lactation persistency. In: Proceedings of the AAABG 17th Conference 2007 (17), pp. 565-568. From: 17th AAABG Conference 2007, 23-26 September 2007, Armidale, NSW, Australia.
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QTL regions associated with lactation persistency and protein yield in sheep have been identified on OAR3 and OAR20. Genotyping of 8 out of 15 existing microsatellite markers on OAR3, and 5 out of 9 on OAR20, in the QTL region of interest, over additional backcross and double backcross daughters, resulted in high resolution mapping of these two QTL regions to 10 cM and 20cM intervals respectively using Linkage Analysis (LA). An additional 28 informative markers (12 on OAR3 and 16 on OAR20) have been genotyped and combined Linkage Analysis/ Linkage Disequilibrium analysis (LA/LD) will be used to obtain further resolution in these regions so that positional candidate genes can be identified. From standard QTL analyses animals with contrasting genotypes ‘Q-’ and ‘q-’ for desired QTLs have been identified for use in functional (transcriptome) studies to obtain a list of differentially expressed genes. The transect of both positional and functional (differentially expressed) genes should lead to a short list of positional functional candidate genes for further analyses and comparative mapping to dairy cows.
|Item Type:||Conference Item (Refereed Research Paper - E1)|
|Keywords:||dairy cattle; selective breeding; genetics; QTL; genomics; quantitative genetics|
Conference theme "Genetic Improvement - Making it Happen"
|Date Deposited:||20 Jul 2010 04:43|
|FoR Codes:||07 AGRICULTURAL AND VETERINARY SCIENCES > 0702 Animal Production > 070201 Animal Breeding @ 50%
06 BIOLOGICAL SCIENCES > 0604 Genetics > 060412 Quantitative Genetics (incl Disease and Trait Mapping Genetics) @ 50%
|SEO Codes:||97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 40%
83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8303 Livestock Raising > 830302 Dairy Cattle @ 60%