Isolation and characterization of 29 microsatellite loci for studies of population connectivity in the butterflyfishes Chaetodon trifascialis and Chaetodon lunulatus

Lawton, Rebecca J., Pratchett, Morgan S., and Bay, Line K. (2010) Isolation and characterization of 29 microsatellite loci for studies of population connectivity in the butterflyfishes Chaetodon trifascialis and Chaetodon lunulatus. Conservation Genetics Resources, 2 (Supplement 1). 209-213.

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Abstract

The highly specialised coral feeding butterflyfish Chaetodon trifascialis is increasingly threatened by the global degradation of coral reef habitats. To reveal the population genetic structure of C. trifascialis across its Indo-Pacific range, primers for thirteen independent microsatellite loci were developed. Observed heterozygosities ranged from 0.174 to 1 and expected heterozygosities ranged from 0.043 to 0.920 in 20 individuals from one Northern Great Barrier Reef location. Primers for 16 independent loci were also developed for the related butterflyfish Chaetodon lunlulatus to enable comparative studies of population genetic structure. These loci had observed heterozygosities ranging from 0.476 to 0.952 and expected heterozygosities ranging from 0.484 to 0.933. Good amplification success and levels of polymorphism were obtained when cross-testing the loci between the two species. These loci will therefore be useful to examine population genetic structure in C. trifascialis and C. lunulatus and possibly other related butterflyfish species.

Item ID: 10154
Item Type: Article (Refereed Research - C1)
Keywords: tropical reef fish; connectivity; microsatellite primers; Chaetodon trifascialis; Chaetodon lunulatus
ISSN: 1877-7260
Funders: ARC Centre of Excellence for Coral Reef Studies, Marine and Tropical Science Research Facility
Date Deposited: 16 Aug 2010 23:21
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
Citation Count from Web of Science Web of Science 5
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